WHAT WE CAN DO
Understanding on how RNA or gene is expressed, will lead to a better understanding of in vivo biological systems. In order to do so, it is imperative to be able to detect and quntify the absolute level of RNA directly from a minute amount of biological sample without RNA purification and reverse transcription. Using QuntiGene reagents based on branched DNA technology combined with state-of-the-art instrumentation, i-DNA has established a high throughput 96-well RNA quantitation platform. This enables i-DNA to go from cell or tissue to quantifying the absolute target RNA level in 24-38 hours turnaround.
TO DO THIS:
RNA gene expression service.
i-DNA offers RNA gene expression service starting from any of these sample types – Cultured Cells, whole blood, PAXgene blood or dried blood spots, fresh/frozen tissues, FFPE samples, purified RNA.
Customer provided the sample and GenBank accession number of target gene.
i-DNA designs specific probe set against the target gene and synthesize it.
i-DNA performs the RNA quantitation and provides customer the date/ report.
Key benefits are:
- Sensitivity comparable to real time PRC, limit of detection ≤ 200 transcripts/ assay well
- RNA quantitation directly from cultured cell or whole blood lysates or fresh, frozen or formalin-fixed, paraffin-embedded (FFPE) tissue homogenates
- No RNA purification
- No reverse transcription
- No target amplification
- Simple assay workflow, significantly reduce turnaround time
- Widely used in biomarker discovery, secondary screening, microarray validation, quantification of RNAi knockdowns and predictive toxicology
Note: QuantiGene bDNA-based gene expression solution demonstrates clear performance benefits over PCR based technologies in an independent FDA MAQC validation study published in Nature Biotechnology 2006, Vol. 24, p.1115-22. Subsequently, there are numerous papers published using QuantiGene. Contact i-DNA for the up-to-date publication list.
Consider outsourcing your RNA gene expression project, please contact i-DNA for a no-obligation consultation. All information provided will be kept strictly confidential.